How is the proper use of sterilization cycles verified?

The proper use of sterilization cycles is verified through periodic use of a Biological Indicator, commonly known as a spore test. This method involves using a test organism that is highly resistant to sterilization processes, such as Bacillus stearothermophilus for steam sterilization. The spore test is placed in the sterilization load and subsequently incubated after the cycle is completed. If the spores are killed, it confirms that the sterilization parameters were met and effective; if they survive, it indicates a failure in the process, signaling the need for further investigation into the sterilization procedures or equipment.

While washing instruments is an important part of the overall infection control process, it does not verify the effectiveness of sterilization. Visual inspections and electronic monitoring systems may assist in ensuring proper protocols are followed but do not provide the definitive validation that the Biological Indicator offers. The spore test provides conclusive evidence about the sterilization process, making it the most reliable method for verifying proper use.